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Biomolecular Research Center Resources
provide the following capabilities and associated equipment:
Imaging
Facilities
 Routine
histology (tissue preparation, sectioning and staining, microtome,
cryostat, and immunohisto-
immunofluorescence) is
accessible to all researchers
Olympus
BX60 Fluorescence microscope
Nikon
Eclipse TS100 Microscope with Fluorescence capabilities
Two
Scanning Electron Microscopes
Kodak
Digital Imaging Station Model 440C
Real
Time PCR Analysis of Alternative Splicing
ABI 7300
and an I-Core Smart Cycler II Real-Time PCR Thermocycler are available
for real time
quantitative PCR
Various
standard PCR thermocyclers (Techne and MJ Research)
LI-COR
Global IR2 4200 gel-based automated DNA sequencer with 64 lane capacity
(upgradeable
to 96 lanes)
DNA and Protein Isolation and Characterization
Sorvall
High Speed Centrifuge
Beckman
TL100 Ultracentrifuge
Omni GLH
Tissue Homogenizer
Savant
SC110A/UVS400 Concentrator/Vacuum System
4 HPLCs
(including size exclusion and reverse phase chromatography) and LC (ion
exchange and
affinity chromatography)
1-D and
2-D gel electrophoresis systems
Protein
electroelution system
Walk-in
cold room and temperature-controlled chromatography cabinet
2
Photodiode array spectrophotometers and 12 CCD array spectrophotometers
(Hewlett-Packard
Model 8452 diode array
Bausch and Lomb 2000)
Mini
Protean 3 Dodeca Cell
Mini
Protean 3 Electrophoresis cell
Trans-blot
semi dry transfer cell w/ PowerPac 200 Power supply
Protean
IEF Cell
Kodak
Image Station 440CF
PDQuest
software
FX Pro
Plus multi-imager (Molecular imager)
Laser for
FX Pro (Molecular imager)
Agfa CP
1000 film processor
Gyromax
737 and 737R Incubators
BeckmanCoulter
Epics XL model flow cytometer
Beckman
scintillation counter
Recombinant protein production (eukaryotic and prokaryotic expression)
Routine laboratory items necessary for cloning and expression of
recombinant proteins are available. Cell/tissue culture facilities
as well as Molecular Biology facilities support the production of
recombinant proteins. Large scale bacterial culture capacity and
sonicator are available. Site-directed mutagenesis capabilities are
established for testing structure/function questions. Large-scale
(100 liters) preps are performed at Utah State University, Logan UT.
Protein structure determination
Molecular
modeling stations (Silicon Graphics Tezro molecular modeling station)
Fluorescence
spectrometer (Ferrand Optical Mark-1)
Jasco
J-810 spectropolarimeter to allow secondary structure determination by
circular dichroism
Beckman
XL-I Analytical ultracentrifuge for velocity and equilibrium
sedimentation analysis
E-500
EleXsys x-band Electron Spin Resonance (ESR) spectrometer for structural
and interaction studies
ThermoElectron
Deca XP-Plus tandem mass spectrometer with Surveyor two-dimensional HPLC
for
Proteomic analysis,
identification of proteins and analysis of posttranslational
modifications
MicroCal
Microcalorimeter for isothermal titrational analysis of protein
interactions
Veeco
Metrology Digital Instruments Nanoscope IV Scanning Probe Microscope for
structure and
interaction analysis is
available in the laboratory of our collaborator
Protein interaction determination
These systems can be used to determine molar mass as well as size and
shape information for the
study of interactions.
Field-flow
fractionation channels (including thermal, electrical and flow FFF)
Multiangle
laser light scatter (MALS) detector in tandem with Size Exclusion
Chromatography
(SEC) and a refractive
index detector
Dynamic
light scatter (DLS) detector for determination of hydrodynamic radius
Yeast Two
Hybrid analysis of interactions in vivo
Reichert
SR7000 Surface Plasmon Resonance spectrometer for real time interaction
analysis
Olis
Stopped flow kinetic Spectroflourimetry
Varian
Cary Fluorescence Spectroscopy
Time-resolved
UV resonance Raman Spectroscopy
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