PHYS 307 -- Intro. to Biophysics : Lab - Sp 2006

(Note: This syllabus is subject to change. Rev. 1/20/06)

Location: SN414      Time: Friday 1:40pm – 4:30pm

Text:   "Phys307 Lab Exercises" by Byung Kim
Instructor:    Dr. Byung Kim    ByungKim@boisestate.edu    (208) 426-3659    MP-425
Office Hours:    MWF 9:30am – 10:30am
GOAL: Enhanced understanding of the molecular scale structures, inter- and intra-molecular interactions, and mechanical properties of biomolecular materials by using a state of the art technique, atomic force microscopy (AFM). The AFM instrumentation will also be introduced. Assessment will come from lab reports.
Week Fri date Topics
     
  Part 1:     Atomic Foce Microscopy
1. 1/20 Introduction to Atomic Force Microscopy (AFM)
2. 1/27 Biological Imaging Modes (Contact mode, Noncontact, Tapping Mode)
3. 2/3 Attractive force, repulsive force, and Adhesion
4. 2/10 Observation of Thermal Motion (Yeast with Optical Microscope)
5. 2/17 Scanning Electron Microscope (SEM)
6. 2/24 Antibody-Antigen Interaction (Force Spectroscopy)
  Part 2:     Biopolymers
7. 3/3 Imaging of DNA fragment using AFM
8. 3/10 Proteins / mica (AFM) and Protein Folding (Force Spectroscopy)
9. 3/17 Collagen structure with Fluorescence Microscope
10. 3/24 Membrane E-Coli (AFM)

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SPRING BREAK 3/27 - 3/31
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  Part 3:     Muscle, Nerve and Visual Systems
11. 4/7 Bacterial System: Yeast, Staphylococcus (AFM)
12. 4/14 Actin Fibril / Microtubule (AFM)
13. 4/21 Cell-Surface Interaction (AFM)
14. 4/28 Neuronal Synapse (AFM)
15. 5/5 Drosophila Eye (AFM)
16. 5/12 No Class