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Lisa Warner, Ph.D.

Lisa Warner

Assistant Professor
Biochemistry
Email: lisawarner@boisestate.edu
Office: SCNC 154D
Phone: (208) 426-2260
Dr. Warner’s Research Group Website

Educational Background

2011: Ph.D. Biochemistry, University of Colorado, Boulder, CO
2002: B.S. Chemistry, Boise State University, Boise, ID

Projects

Research imageProtein and RNA interactions

We use integrated structural biology to tackle questions about the structure and function of proteins, RNAs, and complexes in solution.

 

Research image 2In-cell NMR

Our goal is to develop in-cell NMR techniques in order to build metabolic flux models of carbon utilization in microbes that are important in biofuels/chemicals production. In collaboration with scientists at the National Renewable Energy Laboratory (NREL), we use NMR to detect the conversion of single atom isotope labeled metabolite tracers in living cells.

Structure-based drug design

We work together on teams of researchers aiming to develop next-generation drugs for the treatment of diseases including cancer and heart disease.

Publications

21. Sanchez, C., Voith von Voithenberg, L., Warner, L.R., Lamb, D.C., Sattler, M. (2017) “Effects of fluorophore attachment on protein conformation and dynamics studied by spFRET and NMR.” Chemistry

20. Warner, L.R., Gatzeva-Topalova, P.Z., Doerner, P.A., Pardi, A., and Sousa, M.C. (2017) “Flexibility in the Periplasmic Domain of BamA is Important for Function.” Structure 25:94-106.

19. Mourão, A.*, Bonnal, S.*, Soni, K.*, Warner, L.*, Bordonné, R, Valcárcel, J, Sattler, M. (2017) “Structural basis for the recognition of spliceosomal SmN/B/B’ proteins by the RBM5 OCRE domain in splicing regulation.” eLife, 5:e14707. * equal contribution

18. Xiong, W., Lin, P.P., Magnusson, L., Warner, L., Liao, J.C., Maness P-C., Chou, K.J., (2016) “CO2-fixing one-carbon metabolism in a cellulose-degrading bacterium Clostridium thermocellum” PNAS, 113:13180-13185.

17. Voith von Voithenberg, L., Sanchez, C., Kang, H-S., Madl, T., Zanier, K., Bart, A., Warner, L.R., Sattler, M., Lamb, D.C. (2016) “Recognition of the 3’splice site RNA by the U2AF heterodimer involves a dynamic population shift” PNAS, 113:E7169–E7175.

16. Warner, L.R., Gjersing, E., Follett, S., Elliott, K., Dzyuba, S., Varga, K., (2016) “The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy.” Biochemistry and Biophysics Reports, 8:75–80.

15. Xiao, Y., Warner, L.R., Latham, M.P. Ahn, N.G., Pardi, A. (2015) “Structure-Based Assignment of Ile, Leu, and Val Methyl Groups in the Active and Inactive Forms of the Mitogen-Activated Protein Kinase Extracellular Signal-Regulated Kinase 2.” Biochemistry45 (28): 4307-4319.

14. Hennig, J., Warner, L.R., Simon, B., Geerlof, A., Mackereth, C.D., Sattler, M., (2015) “Structural Analysis of Protein–RNA Complexes in Solution Using NMR Paramagnetic Relaxation Enhancements.” Methods in Enzymology, 558:333-362.

13. Voith von Voithenberg, L., Sanchez, C., Warner, L., Zhang, Y., Sattler, M., Lamb, D.C. (2014) “Conformational Dynamics During Spliceosome Assembly Investigated by Single-Pair FRET.” Biophysical Journal106 (2): 465a.

12. Huang, J., Warner L.R., Sanchez, C., Gabel, F., Madl, T., Mackereth, C.D., Sattler, M., Blackledge, M., (2014) “Mapping the dynamic conformational equilibrium of the multi-domain splicing factor U2AF65 using NMR and SAXS.” J. Amer. Chem. Soc.136 (19): 7068-7076.

11. Schilling, F., *Warner L.R.*, Skinner, T., Sattler, M., Glaser, S.J., (2014) “Next Generation Heteronuclear Decoupling for High Field Biomolecular NMR.” Angew. Chem. Int. Ed. Engl.126 (17): 4564-4568. *equal contribution

10. Xiao Y., Lee T., Latham M.P., Warner L.R.,Tanimoto A., Pardi A., Ahn N.G. (2014) “Phosphorylation Releases Constraints to Domain Motion in ERK2.” PNAS111 (7): 2506-2511.

9. Chapnick D.A., Warner L., Bernet J., Rao T., Liu X. (2011) Partners in crime: the TGFβ and MAPK pathways in cancer progression. Cell & Bioscience2011 1:42.

8. Warner, L.R.*, Varga, K.*, Lange, O.F., Baker, S.L., Baker, D., Sousa, M.C., Pardi, A. (2011) “Structure of the BamC two-domain protein obtained by Rosetta with a limited NMR data set.” J. Mol. Bio.411: 83-95. *equal contribution

7. Gatzeva-Topalova, P.Z., Warner, L.R., Pardi, A., and Sousa, M.C. (2010) “Structure and flexibility of the complete periplasmic domain of BamA: the protein insertion machine of the outer membrane.” Structure18: 1492-1501.

6. Yingst, S., Bloxham, K., Warner, L.R., Brown, R.J., Cole, J., Kenoyer, L., Knowlton, W.B., Oxford, J.T. (2009) “Characterization of collagenous matrix assembly in a chondrocyte model system.” J. Biomed. Mater. Res. A90: 247-255.

5. Dufty, B.M., Warner, L.R., Hou, S.T., Jiang, S.X., Gomez-Isla, T., Leenhouts, K.M., Oxford, J.T., Feany, M.B., Masliah, E., and Rohn, T.T. (2007) “Calpain-cleavage of alpha-synuclein: connecting proteolytic processing to disease-linked aggregation.” Am. J. Pathol.170: 1725-1738.

4. Warner, L.R., Blasick, C.M., Brown, R.J., and Oxford, J.T. (2007) “Expression, purification, and refolding of recombinant collagen alpha1(XI) amino terminal domain splice variants.” Protein Expr. Purif.52: 403-409.

3. Warner, L.R., Brown, R.J., Yingst, S.M., and Oxford, J.T. (2006) “Isoform-specific heparan sulfate binding within the amino-terminal noncollagenous domain of collagen alpha1(XI).” J. Biol. Chem.281: 39507-39516.

2. Fallahi, A., Kroll, B., Warner, L.R., Oxford, R.J., Irwin, K.M., Mercer, L.M., Shadle, S.E., and Oxford, J.T. (2005) “Structural model of the amino propeptide of collagen XI alpha1 chain with similarity to the LNS domains.” Protein Sci.14: 1526-1537.

1. Charlier, H.A., Jr., Albertson, C., Thornock, C., Warner, L., Hurst, T., and Ellis, R. (2005) “Comparison of the effects of arsenic (V), cadmium (II), and mercury (II) single metal and mixed metal exposure in radish, Raphanus sativus, fescue grass, Festuca ovina, and duckweed, Lemna minor.”Bull Environ Contam Toxicol75: 474-481.

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