Lisa Warner, Ph.D.
The overarching theme of our research is understanding the relationship between the structures and dynamics of biomolecules and their functions. The subject of our studies has centered on several individual and multidomain proteins, peptides, and nucleic acids and their complexes. We use techniques including solution, solid state, and high-resolution magic angle spinning nuclear magnetic resonance (NMR, ssNMR, and HR-MAS NMR) and small angle scattering (SAS) to solve structures, analyze dynamics, and model conformational sampling and ensembles of multi domain proteins.
Phone: (208) 426-2260
Biomolecular Research Center
Boise State University
1910 University Dr.
Boise, ID 83725-1515
|Dates||Degree and Institution|
|Aug 2006 – Dec 2011||Ph.D. Biochemistry, University of Colorado, Boulder|
|Aug 1997 – May 2002||B.S. Chemistry, Boise State University|
|Dates||Position and Institution|
|August 2018- Current||Assistant Professor, Department of Chemistry and Biochemistry
Boise State University, Boise, ID, USA
|Oct 2015 – July 2018||Assistant Research Professor
Boise State University, Boise, ID, USA
Conducted independent research characterizing protein-protein, protein-RNA, and protein-small molecule interactions. Successfully competed for and was awarded NSF and NIH grants. Managed biophysical characterization equipment and protein expression and purification facility.
|Dec 2014 – Sept 2015||Postdoctoral Researcher
National Renewable Resource Laboratory, Golden, CO, USA
Characterized the structure of proteins and whole plant tissue involved in biofuel/biomaterials production using solution and solid-state NMR. Worked with a team to track CO2 metabolism in cellulose-degrading bacterium Clostridium thermocellum.
|Apr 2012 – Oct 2014||EMBO Postdoctoral Fellow
Helmholtz Zentrum München/Techniche Universität München
Characterized the structure and dynamics of RNA binding proteins involved in the regulation of alternative splicing using solution NMR. Worked on a team to enhance bioNMR capabilities at high field.
|Jan 2012 – Apr 2012|
Aug 2006 – Dec 2011
University of Colorado, Boulder
Structurally and dynamically characterized proteins of the E. coli β-barrel assembly machine and the MAP kinase ERK2. Incorporated the use of sparse NMR data into non-traditional NMR structure determination.
|May 2004 – Aug 2006||Research Associate
Boise State University
Characterized the biophysical and biochemical properties of the amino terminal domain of type XI collagen α1. Worked on a team to develop AFM imaging of the extracellular matrix secreted by adherent cells.
|Jan 2003 – May 2004||Research Associate
Boise State University
Developed a sensor for benzene recognition in ground water using polymer-assisted molecular recognition.
|May 2002 – Jan 2003||Staff Scientist
Sapidyne Instruments Inc
Developed and maintained KinExA instruments used to measure binding affinities of strong antibody/antigen and protein/protein interactions. Performed instrument installations and trainings at customer sites.
Honors and Awards
|Award||Institution and Date|
|EMBO Long-Term Fellowship ALTF 1520-2011||Helmholtz Zentrum München, Germany 2012-2014|
|NIH NRSA T32 Institutional Training Grant GM008759||University of Colorado, Boulder 2006-2008|
|Isotec sponsorship to attend ICMRBS||2008, 2010|
|Chemistry Teaching Award||University of Colorado, Boulder, 2006|
|Sigma Xi Grants in Aid of Research||Boise State University, 2004|
|Dean’s List with High Honors||Boise State University, 2001-2002|
|Analyst Certification in Analytical Chemistry||Boise State University, 2001|
|Irving Award for Excellence in Organic Chemistry||Boise State University, 1999|
- Sanchez, C., Voith von Voithenberg, L., Warner, L.R., Lamb, D.C., Sattler, M. (2017) “Effects of fluorophore attachment on protein conformation and dynamics studied by spFRET and NMR.” Chemistry (Epub ahead of print)
- Warner, L.R., Gatzeva-Topalova, P.Z., Doerner, P.A., Pardi, A., and Sousa, M.C. (2017) “Flexibility in the Periplasmic Domain of BamA is Important for Function.” Structure 25, 94-106.
- Mourão, A.*, Bonnal, S.*, Soni, K.*, Warner, L.*, Bordonné, R, Valcárcel, J, Sattler, M. “Structural basis for the recognition of spliceosomal SmN/B/B’ proteins by the RBM5 OCRE domain in splicing regulation.” eLife, 5, e14707. * equal contribution
- Xiong, W., Lin, P.P., Magnusson, L., Warner, L., Liao, J.C., Maness P-C., Chou, K.J., (2016) “CO2-fixing one-carbon metabolism in a cellulose-degrading bacterium Clostridium thermocellum” PNAS, 113, 13180-13185.
- Voith von Voithenberg, L., Sanchez, C., Kang, H-S., Madl, T., Zanier, K., Bart, A., Warner, L.R., Sattler, M., Lamb, D.C. “Recognition of the 3’splice site RNA by the U2AF heterodimer involves a dynamic population shift” PNAS, 113, E7169–E7175.
- Warner, L.R., Gjersing, E., Follett, S., Elliott, K., Dzyuba, S., Varga, K., (2016) “The effects of high concentrations of ionic liquid on GB1 protein structure and dynamics probed by high-resolution magic-angle-spinning NMR spectroscopy.” Biochemistry and Biophysics Reports 8, 75–80.
- Xiao, Y., Warner, L.R. Latham, M.P. Ahn, N.G., Pardi, A. (2015) “Structure-Based Assignment of Ile, Leu, and Val Methyl Groups in the Active and Inactive Forms of the Mitogen-Activated Protein Kinase Extracellular Signal-Regulated Kinase 2.” Biochemistry 45 (28): 4307-4319.
- Hennig, J., Warner, L.R., Simon, B., Geerlof, A., Mackereth, C.D., Sattler, M., (2015) “Structural Analysis of Protein–RNA Complexes in Solution Using NMR Paramagnetic Relaxation Enhancements.” Methods in Enzymology 558: 333-362.
- Voith von Voithenberg, L., Sanchez, C., Warner, L., Zhang, Y., Sattler, M., Lamb, D.C. (2014) “Conformational Dynamics During Spliceosome Assembly Investigated by Single-Pair FRET.” Biophysical Journal 106 (2): 465a.
- Huang, J., Warner L.R., Sanchez, C., Gabel, F., Madl, T., Mackereth, C.D., Sattler, M., Blackledge, M., (2014) “Mapping the dynamic conformational equilibrium of the multi-domain splicing factor U2AF65 using NMR and SAXS.” Amer. Chem. Soc. 136 (19): 7068-7076.
- Warner L.R.*, Schilling, F.*, Skinner, T., Sattler, M., Glaser, S.J., (2014) “Next Generation Heteronuclear Decoupling for High Field Biomolecular NMR.” Chem. Int. Ed. Engl. 126 (17): 4564-4568. *equal contribution
- Xiao Y., Lee T., Latham M.P., Warner L.R., Tanimoto A., Pardi A., Ahn N.G. (2014) “Phosphorylation Releases Constraints to Domain Motion in ERK2.” PNAS 111 (7): 2506-2511.
- Chapnick D.A., Warner L., Bernet J., Rao T., Liu X. (2011) Partners in crime: the TGFβ and MAPK pathways in cancer progression. Cell & Bioscience 2011 1:42.
- Warner, L.R.*, Varga, K.*, Lange, O.F., Baker, S.L., Baker, D., Sousa, M.C., Pardi, A. (2011) “Structure of the BamC two-domain protein obtained by Rosetta with a limited NMR data set.” Mol. Bio. 411: 83-95. *equal contribution
- Gatzeva-Topalova, P.Z., Warner, L.R., Pardi, A., and Sousa, M.C. (2010) “Structure and flexibility of the complete periplasmic domain of BamA: the protein insertion machine of the outer membrane.” Structure 18: 1492-1501.
- Yingst, S., Bloxham, K., Warner, L.R., Brown, R.J., Cole, J., Kenoyer, L., Knowlton, W.B., Oxford, J.T. (2009) “Characterization of collagenous matrix assembly in a chondrocyte model system.” Biomed. Mater. Res. A 90: 247-255.
- Dufty, B.M., Warner, L.R., Hou, S.T., Jiang, S.X., Gomez-Isla, T., Leenhouts, K.M., Oxford, J.T., Feany, M.B., Masliah, E., and Rohn, T.T. (2007) “Calpain-cleavage of alpha-synuclein: connecting proteolytic processing to disease-linked aggregation.” J. Pathol. 170: 1725-1738.
- Warner, L.R., Blasick, C.M., Brown, R.J., and Oxford, J.T. (2007) “Expression, purification, and refolding of recombinant collagen alpha1(XI) amino terminal domain splice variants.” Protein Expr. Purif. 52: 403-409.
- Warner, L.R., Brown, R.J., Yingst, S.M., and Oxford, J.T. (2006) “Isoform-specific heparan sulfate binding within the amino-terminal noncollagenous domain of collagen alpha1(XI).” Biol. Chem. 281: 39507-39516.
- Fallahi, A., Kroll, B., Warner, L.R., Oxford, R.J., Irwin, K.M., Mercer, L.M., Shadle, S.E., and Oxford, J.T. (2005) “Structural model of the amino propeptide of collagen XI alpha1 chain with similarity to the LNS domains.” Protein Sci. 14: 1526-1537.
- Charlier, H.A., Jr., Albertson, C., Thornock, C., Warner, L., Hurst, T., and Ellis, R. (2005) “Comparison of the effects of arsenic (V), cadmium (II), and mercury (II) single metal and mixed metal exposure in radish, Raphanus sativus, fescue grass, Festuca ovina, and duckweed, Lemna minor.” Bull Environ Contam Toxicol 75: 474-481.