The 4D Living Genome
Over the last few decades great advances have been made in our understanding of gene expression and the human genome. In 2003 the human genome was sequenced for the first time, and while this was a great advance, it created more questions than it answered. Recent studies have shown that the DNA code is important, but the 4D (spatiotemporal) organization and dynamics of the living genome plays an equally critical role in how genes get accessed and expressed. Additionally, the temporal coordination of transcription factors and gene activation adds another layer of complexity. My project focuses on addressing both the spatial and temporal aspects of the genome through cutting edge microscopy technique known as 3D Orbital Tracking Fluorescence Correlation Spectroscopy (3DOT-FCCS) in conjunction with Molecular Dynamics simulations. The synergistic use of these techniques will provide a clearer picture of the rules that govern the human genome.